shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.
shows that taxonomy prediction accuracy is <50% for V4 sequences.
shows low accuracy of closed- and open-ref. QIIME OTUs.
shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.
UPARSE tutorial video
posted on YouTube. Make OTUs from MiSeq reads.
New in v11
OTU errors and biases
Sequence alignment commands
Chimera detection and filtering
Sequence, tree and graph-based clustering
Commands for diversity analysis
Commands for reads in FASTQ format
Commands for sequences in FASTx format (FASTA and FASTQ)
Machine learning and finding informative OTUs
Commands for OTU analysis and denoising
OTU table commands
Sequence database search
Labels and annotations