Next-generation reads
fastq_chars Report frequencies of Q score ASCII characters in FASTQ file
fastq_eestats Report quality/e.e. per position for reads in FASTQ file
fastq_eestats2 Report number of reads retained at difference length and e.e. cutoffs
fastq_filter Filter reads in FASTQ file by e.e. and other criteria
fastq_join Concatenate forward (R1) and reverse (R2) paired reads
fastq_mergepairs Assemble (merge) paired reads
fastq_sra_splitpairs Recover paired reads from SRA interleaved or concatenated format
fastx2qiime Convert sample labels from usearch to QIIME format
fastx_demux Assign reads to samples (demultiplex)
fastx_findorfs Identify ORFs in nucleotide sequences
fastx_get_sample_names Extract sample names from FASTx file
fastx_info Report summary information about a FASTx file
fastx_learn Estimate error rates from amplicon reads
fastx_subsample Extract random sub-sample from FASTx file
fastx_syncpairs Sort forward and reverse reads into the same order
fastx_trim_primer Remove primer-binding sequence from FASTx file
fastx_truncate Truncate sequences in FASTx file
filter_lowc Filter low-complexity sequences from FASTx file
filter_phix Remove PhiX spike sequences from FASTx file
search_oligodb Search for matches to short nucleotide sequences, e.g. primers
search_pcr In-silico PCR, search for matches to pairs of primers in database
search_pcr2 In-silico PCR, search for matches to primer pair
search_phix Search for matches to PhiX sequence