Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Taxonomy commands

  calc_lcr_probs  Calculate Lowest Common Rank probabilities from dist. matrix with taxonomy
  makeudb_sintax  Make UDB database file for sintax
  nbc_tax  Predict taxonomy using RDP Naive Bayesian Classifier algorithm
  sintax  Predict taxonomy using SINTAX algorithm
  sintax_summary  Generate summary report from sintax output