Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Sequence, tree and graph-based clustering

  closed_ref  Make OTU table using closed-reference clustering
  cluster_aggd  Cluster distance matrix using agglomerative clustering
  cluster_edges  Find connected components of graph (single-linkage clustering)
  cluster_fast  Cluster sequences using UCLUST
  cluster_otus  Cluster sequences using UPARSE
  cluster_smallmem  Cluster sequencees using UCLUST
  cluster_tree  Construct clusters from tree using distance cutoff