Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Chimera detection and filtering

  annot  Annotate OTU sequences as known (mock or large ref. db.), chimeric etc.
  uchime2_ref  Chimera search using UCHIME2 algorithm
  uchime3_denovo  Chimera search using UCHIME3 de-novo algorithm
  unoise3  Denoise amplicon reads