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Searching query structure(s) against a database
Pairwise alignment
Structure superposition
Output files
Alignments and hit summaries
Structure files
File formats for structures and search databases
STRUCTS
Specifying structure file(s) on the command line
Converting
How to convert between structure file formats
getchains
Extract one or more chains from a structure(s) file
Resources
Scaling to big database searches
E-values
What is an E-value and what threshold should you set?
SCOP40
About SCOP40 benchmark and test results
Figures
How to make high-quality protein structure alignment figures
Time, memory and disk space
Case study: building and running PDB-95
Prebuilt databases
in .bca format including SCOP40, PDB and AFDB
Video talks
on 16S data analysis posted.
URMAP
ultra-fast read mapper posted (
paper)
.
~20% of taxonomy annotations in SILVA and Greengenes are wrong (
paper
).
Taxonomy prediction is <50% accurate for 16S V4 sequences (
paper
).
97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (
paper
).