Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Operational Taxonomic Units (OTUs)

  Defining and interpreting OTUs
  Mapping reads to OTUs
  Closed-reference OTUs
  Problems with closed- and open-reference OTUs
  OTU accuracy
  How to compare two sets of OTUs
  How to make OTUs from different studies or sequencing runs
  Spurious OTUs in mock and real samples
  USEARCH, fast search by global alignment
  UBLAST, fast search by local alignment
  UPARSE, OTUs from next-gen amplicon reads
  UNOISE, denoise / error-correction for amplicon reads
  UPARSE-REF, amplicon read parsing by parsimony
  SEARCH_16S, find 16S rRNA genes in chromosomes and contigs
  SINAPS, predict traits from 16S sequence
  UCLUST, general-purpose sequence clustering
  UCHIME, chimera search
  UCHIME2, improved chimera search
  UNBIAS, correct abundance bias in OTU table
  UNCROSS2, detect and filter cross-talk in OTU table
  Closed-reference OTUs
  RDP Naive Bayesian Classifier