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11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.



UNCROSS2 algorithm

See also
  otutab_xtalk command

For description of the algorithm, see paper

Reference (please cite)
R.C. Edgar (2018), UNCROSS2: identification of cross-talk in 16S rRNA OTU tables, https://doi.org/10.1101/400762
  • Cross-talk rate is approx. 1% in many Illumina datasets

  • Cross-talk can cause false positive core microbiome

  • UNCROSS2 algorithm for filtering cross-talk