Home Software Services About Contact     
Follow on twitter

Robert C. Edgar on twitter

11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.



Topics by category

  Rarefaction curves and subsampling
  Sequence comparison and alignment
  Sequence database search
  Read quality filtering
  Paired read assembly (merging pairs into contigs)
  Errors and biases in amplicon sequencing
  File formats
  Output files
  Operational Taxonomic Units (OTUs)
  Parameters and options
  USEARCH software installation and use
  Commentaries and rebuttals
  Benchmark tests
  Publications and citing USEARCH
  OTU analysis
  Machine learning
  Designing 16S experiments