for visualizing alpha diversity.
shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.
shows that taxonomy prediction accuracy is <50% for V4 sequences.
shows low accuracy of closed- and open-ref. QIIME OTUs.
shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.
UPARSE tutorial video
posted on YouTube. Make OTUs from MiSeq reads.
New in v11
OTU errors and biases
Commentaries and rebuttals
QIIME v1 grossly over-estimates mock community diversity
Analysis of Brown 2017 ITS mock data
Problems with CD-HIT sequence identities
Problems with closed- and open-reference OTUs
Westcott & Schloss MCC OTU metric
Rebuttal to Dr. P. Schloss's comments on OTU identity
Rebuttal to ARB / SILVA team's comments
Comments on Westcott & Schloss 2017 OptiClust