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11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.



Commentaries and rebuttals

  QIIME v1 grossly over-estimates mock community diversity
  Analysis of Brown 2017 ITS mock data
  Problems with CD-HIT sequence identities
  Problems with closed- and open-reference OTUs
  Westcott & Schloss MCC OTU metric
  Rebuttal to Dr. P. Schloss's comments on OTU identity
  Rebuttal to ARB / SILVA team's comments
  Comments on Westcott & Schloss 2017 OptiClust