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11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.




  Diversity overview
  Alpha diversity
  Alpha diversity metrics
  Beta diversity
  Beta diversity metrics
  Why use UniFrac?
  Interpreting alpha and beta diversity
  Recommended alpha and beta
  Using diversity metrics to compare groups
  Significance of diversity differences between groups
  Jost's effective number of species