Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Size annotations

See also
Annotations
fastx_strip_annots command

Size annotations are used to indicate cluster sizes of representative sequences.

A size annotation is specified as a size=N field in the sequence label. It may appear anywhere in the label. It must be delimited by semi-colons, which may optionally be omitted at the end of a label, though this is not recommended.

You can propagate size annotations through multiple commands by using the -sizein and -sizeout options, which are supported by most (but not all) clustering commands.

Examples

>KR08766;size=1;

>KR08766;size=2

>READ-127;size=5;sample=soil3;

@M141:79:749142:1:1101:14941:1421;size=57721;