Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

fastx_strip_annots command

This command strips all usearch-style annotations from sequence labels by deleting everything after the first semi-colon (;).

Input can be in FASTA or FASTQ format. The output filename is specified by -fastqout (FASTQ format) and / or -fastaout (FASTA format). You cannot create FASTQ output from FASTA input.

Example

usearch -fastx_strip_annots seqs.fa -fastaout seqs_without_annots.fa