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11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

24-Nov-2016
UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.

 

USEARCH v11
 New in v11 

tabbed2otutab command

Converts tabbed text file to an OTU table.

Each line in the tabbed text file contains two fields: #1. SeqLabel, #2. OTUlabel.

The SeqLabel field must contain a sample name.

The -output optoin specifies the OTU table filename.

Example

usearch -tabbed2otutab hits.txt -output otutab.txt