Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

-strand option

Required for nucleotide searches. Value must be set to plus or both.

-strand plus		Search for hits on the forward (plus) strand only. This is faster, so is recommended when the query sequences are known to be oriented on the same strand as the database.
-strand both		Search for hits on both the forward and reverse-complemented strands.