Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

-output_no_hits option

Specifies that a record should be written to search or clustering output files if there are no hits for the query sequence. By default, no records are written except for uc files, which always have N (no-hit) records for unmatched queries.

A star (*) is written in place of the target label. Placeholders (zeroes or empty values) are written in other fields of the record.