Home Software Services About Contact usearch manual
fastx_revcomp command
Commands > FASTA/Q files, Reads

Reverse-complements sequences in a FASTA or FASTQ file.

Output is written to -fastaout (FASTA) and/or -fastqout (FASTQ). You cannot use -fastqout if the input is FASTQ because the quality scores are not known.

The -label_suffix option specifies a string that is appended to the labels in the output file(s).


usearch -fastx_revcomp reads.fastq -label_suffix _RC -fastaout reads_rc.fa