Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

fastx_revcomp command

Reverse-complements sequences in a FASTA or FASTQ file.

Output is written to -fastaout (FASTA) and/or -fastqout (FASTQ). You cannot use -fastqout if the input is FASTQ because the quality scores are not known.

The -label_suffix option specifies a string that is appended to the labels in the output file(s).

Example

usearch -fastx_revcomp reads.fastq -label_suffix _RC -fastaout reads_rc.fa