Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

ROC curve

See Wikipedia article on ROC curves.

Fawcett 2006 is an excellent introduction to ROC analysis.

Reference
T. Fawcett (2006), An introduction to ROC analysis, Pattern Recognition Letters 27(8) 861-874. https://doi.org/10.1016/j.patrec.2005.10.010