Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

How to set the $usearch environment variable

The example pipelines require that you set an environment variable called $usearch with the name of your usearch command.

Usearch is distributed as a single binary (executable) file. The usearch binary filename will be something like usearch10.0.786_i86linux32. I will assume that your user name is alice and the usearch binary file stored in your home directory, so the full path name is:

/home/alice/usearch10.0.786_i86linux32

To set the $usearch environment variable, use the export command in bash:

export usearch=/home/alice/usearch10.0.786_i86linux32

Note there is no $ in this command, and there must not be any spaces around the equals sign.

You can put this command into the .bash_profile file in your home directory, then it will be executed every time you start a new shell. Note there is a period at the start of the file name.