Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Low correlation between read abundance and species abundance

Species frequencies determined by shotgun sequencing (top-left) and 16S read frequencies (bottom-left). The Pearson correlation between the freqency distributions is 0.17 (right). Figure from UNBIAS paper.