Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

OTU QC: Low-complexity sequence

Low-complexity reads may be generated during sequencing. For example, Illumina reads typically contain low-complexity noise if they continue past the reverse sequencing adapter due to a short construct. Low-complexity sequence can be detected and filtered using the filter_lowc command.