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Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).
 

USEARCH v11
USEARCH
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UC output file

 
USEARCH cluster format (UC) is a tab-separated text file. UC output is supported by clustering and database search. By convention, the .uc filename extension is used. Each line is either a comment (starts with #) or a record. Every input sequence generates one record (H, S or N); additional record types give information about clusters. If an input sequence matched a target sequence, then the alignment and the identity computed from that alignment are also provided. Fields that do not apply to a given record type are filled with an asterisk placeholder (*).
 
Field   Description
1   Record type S, H, C or N (see table below).
2   Cluster number (0-based).
3   Sequence length (S, N and H) or cluster size (C).
4   For H records, percent identity with target.
5   For H records, the strand: + or - for nucleotides, . for proteins.
6   Not used, parsers should ignore this field. Included for backwards compatibility.
7   Not used, parsers should ignore this field. Included for backwards compatibility.
8   Compressed alignment or the symbol '=' (equals sign). The = indicates that the query is 100% identical to the target sequence (field 10).
9   Label of query sequence (always present).
10   Label of target sequence (H records only).
 
Record   Description
H   Hit. Represents a query-target alignment. For clustering, indicates the cluster assignment for the query. If -maxaccepts > 1, only there is only one H record giving the best hit. To get the other accepts, use another type of output file, or use the -uc_allhits option (requires version 6.0.217 or later).
S   Centroid (clustering only). There is one S record for each cluster, this gives the centroid (representative) sequence label in the 9th field. Redundant with the C record; provided for backwards compatibility.
C   Cluster record (clustering only). The 3rd field is set to the cluster size (number of sequences in the cluster) and the 9th field is set to the label of the centroid sequence.
N   No hit (for database search without clustering only). Indicates that no accepts were found. In the case of clustering, a query with no hits becomes the centroid of a new cluster and generates an S record instead of an N record.