Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

OTU validation data and commands

The HMP mock communities and sequencing protocols are described at HMPDACC.

Titanium reads
I used reads obtained with V3-V5 primers only.

Set	Community	Link to data
Even1P	Even	SRR053818
Even2P	Even	SRR072220
Even3P	Even	SRR072239
Stag1P	Staggered	SRR072221
Stag2P	Staggered	SRR072223
Stag3P	Staggered	SRR072237

MiSeq reads
The MiSeq reads are from Bokulich et al., 2013. Links to the data can be found via the QIIME resources page.

Software and commands

Package	Version	Commands
USEARCH	6.1.351_i86linux32	USEARCH command-lines.
mothur	1.27.0, Linux 64-bit	mothur script.
QIIME	1.5.0 and 1.5.0-dev	QIIME commands.