blast6out file
The blast6out option specifies an output file in a format compatible with the NCBI BLAST -m8 and NCBI BLAST+ -outfmt 6 formats. It is a tab-separated text file with one line per alignment. By convention I use the .b6 extension for files in this format. There are twelve fields, as shown in the table.
| Field | Description | |
| 1 | Query label . | |
| 2 | Target (database sequence or cluster centroid) label . | |
| 3 | Percent identity . | |
| 4 | Alignment length. | |
| 5 | Number of mismatches. | |
| 6 | Number of gap opens. | |
| 7 | Start position in query. Query coordinates start with 1 at the first base in the sequence as it appears in the input file. For translated searches (nucleotide queries, protein targets), query start<end for +ve frame and start>end for -ve frame. | |
| 8 | End position in query. | |
| 9 | Start position in target. Target coordinates start with 1 at the first base in sequence as it appears in the database. For untranslated nucleotide searches, target start<end for plus strand, start>end for a reverse-complement alignment. | |
| 10 | End position in target. | |
| 11 | E-value calculated using Karlin-Altschul statistics . | |
| 12 | Bit score calculated using Karlin-Altschul statistics. |
