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fa2efa command

Example

muscle -fa2efa fasta_filenames.txt -output ensemble.efa

Description

The fa2efa command creates one ensemble FASTA (EFA) file from one or more FASTA files. Input filenames or pathnames are specified in the text file fasta_filenames.txt, one per line.

120,000 new RNA virus species discovered by mining the SRA (paper).

Syncmers are better than minimizers (paper).

Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).