See also
Chimera benchmark home
UPARSE-OTU algorithm with chimera filtering (cluster_otus
command).
UCHIME and UPARSE compared for OTU clustering
Below is Fig 1(a) from
the UPARSE paper.
Magenta
segments in the pie chart show the fraction of chimeric OTUs generated by
several algorithms on a mock community test. The QIIME and mothur pipelines used
UCHIME for chimera detection. Notice that even after UCHIME filtering, about 50%
of the OTUs generated by QIIME and mothur are chimeric, while UPARSE
successfully filters all chimeras from Illumina reads and leaves only one
chimeric OTU with 454 reads.
