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Unbias reference databases

See also
  UNBIAS algorithm
  unbias command

unbias_dbs_v10.0.tar.gz (4.3 Mb)

Copy number reference (copynr.fa)
Contains full-length 16S sequences. A typical label is:

>U28;copynr=3;sp=Campylobacter coli;

The annotation format should be self-explanatory. This database was created by downloading all finished microbial genomes from Genbank and predicting 16S genes using the search_16s command. For each genome, the number of 16S genes was counted.

V4 primer mismatch database (v4diffs.fa)
Contains V4 sequences. A typical label is:

>U118;v4diffs=0;sp=Bacillus cereus;

The v4diffs annotation gives the total number of mismatches with V4F (GTGCCAGCMGCCGCGGTAA) and V4R (GGACTACHVGGGTWTCTAAT). Mismatches were measured using the search_pcr command. If you need reference data for a different primer pair, let me know.

The unbias reference databases were created by Robert C. Edgar and are licensed under a Creative Commons Attribution-ShareAlike 3.0 Unported License.

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