Home Software Services About Contact usearch manual

 

Chimera detection benchmarks

See also
 
UPARSE benchmark home

Algorithm comparisons
   UCHIME vs. ChimeraSlayer
   UCHIME vs. Perseus (AmpliconNoise)
   UCHIME vs. DECIPHER
   UPARSE-OTU (cluster_otus) vs. UCHIME

Conclusion
The UPARSE-OTU algorithm (implemented in the cluster_otus command) is currently the most effective chimera filter for OTU clustering.



Fig 1(a) from the UPARSE paper. Magenta segments in the pie chart show the fraction of chimeric OTUs generated by several algorithms on a mock community test. The QIIME and mothur pipelines used UCHIME for chimera detection. Notice that even after UCHIME filtering, about 50% of the OTUs generated by QIIME and mothur are chimeric, while UPARSE successfully filters all chimeras from Illumina reads and leaves only one chimeric OTU with 454 reads.