| Field | Description | |
| 1 | Query label. | |
| 2 | Query start position (one-based relative to the start of the plus strand, even if the hit is reverse-complemented). | |
| 3 | Query end position (one-based relative to the start of the plus strand, even if the hit is reverse-complemented). | |
| 4 | Query sequence length. | |
| 5 | Label of first primer. | |
| 6 | Strand of first primer match. | |
| 7 | Alignment of first primer in "dot" format. Letters that match the primer are shown as dots, letters indicate query sequence letters that do not match. | |
| 8 | Label of second primer. | |
| 9 | Strand of second primer match.. | |
| 10 | Alignment of second primer in "dot" format (see field 7). | |
| 11 | Amplicon length. | |
| 12 | Amplicon sequence, i.e. the segment of the query sequence that matches the primer. The letters that match the primers are included. |