The input file must contain estimated amplicons with abundances specified by size annotations.

Multithreading is not supported.

Output options are ‑uchimeout, ‑uchimealns, ‑chimeras and ‑nonchimeras. The ‑uchimeout5 option sets the uchimeout format to be compatible with previous versions.

The ‑abskew option sets the abundance skew, which defaults to 2.0.

Example

usearch -uchime_denovo amplicons.fasta -uchimeout results.uchime