An E-value threshold must be specified.

A database file must be specified using the ‑db option.

The ‑strand option is required for nucleotide databases.

Nucleotide, protein and translated searches are supported.

Server mode is supported in v6.0.178 and later.

The -accel option tunes search speed against sensitivity. Smaller values are faster but tend to have lower sensitivity to hits with lower sequence identity. Default is 0.8. See UBLAST algorithm for more discussion.

See also:
  Standard output file options
  Accept options
  Weak hits (Weak hits are especially useful with ublast)
  Termination options
  Indexing options
  Multithreading
  Masking options
  Alignment parameters
  Alignment heuristics

Example

usearch -ublast reads.fasta -db proteins.udb -evalue 1e-9 -accel 0.5 -alnout results.aln