| Field |
|
Description |
| 1 |
|
Query label. |
| 2 |
|
Query start position (one-based relative to the start of the plus strand, even if the hit is reverse-complemented). |
| 3 |
|
Query end position (one-based relative to the start of the plus strand, even if the hit is reverse-complemented). |
| 4 |
|
Query sequence length. |
| 5 |
|
Label of first primer. |
| 6 |
|
Strand of first primer match. |
| 7 |
|
Alignment of first primer in "dot" format. Letters that match the primer are shown as dots, letters indicate query sequence letters that do not match. |
| 8 |
|
Label of second primer. |
| 9 |
|
Strand of second primer match.. |
| 10 |
|
Alignment of second primer in "dot" format (see field 7). |
| 11 |
|
Amplicon length. |
| 12 |
|
Amplicon sequence, i.e. the segment of the query sequence that matches the primer. The letters that match the primers are included. |
| 13 |
|
Number of differences with first primer. |
| 14 |
|
Number of differences with second primer. |
