1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 256k 512k 1m 2m 0 10 20 30 40 50 60 OTUs Combined 20 samples, 257 OTUs, 4.0M reads With singletons Strong noise, 21 OTUs Weak noise, 6 OTUs Low / no noise, 230 OTUs 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70015_A, 39 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70015_M, 27 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70034_A, 60 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70034_M, 37 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70037_A, 39 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70037_M, 48 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70088_A, 41 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70088_M, 40 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70110_A, 36 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70110_M, 46 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70118_A, 28 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70118_M, 50 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70148_A, 72 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70148_M, 39 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70197_A, 39 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70197_M, 34 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70215_A, 35 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70215_M, 50 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70464_A, 35 OTUs, 200.0k reads 1 2 4 8 16 32 64 128 256 512 1k 2k 4k 8k 16k 32k 64k 128k 0 2 4 6 8 10 12 14 16 OTUs 70464_M, 66 OTUs, 200.0k reads With singletons Strong cross-talk Weak cross-talk Strong noise Weak noise Low / no noise