for visualizing alpha diversity.
shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.
shows that taxonomy prediction accuracy is <50% for V4 sequences.
shows low accuracy of closed- and open-ref. QIIME OTUs.
shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.
UPARSE tutorial video
posted on YouTube. Make OTUs from MiSeq reads.
New in v11
OTU errors and biases
A rather obscure name for finding the set of unique sequences. Or, equivalently, the process of finding duplicated (replicate) sequences. See