Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

fasta_explode command

The fasta_explode command creates one FASTA file for each sequence in the input file, which must be in FASTA format. Output file names are the sequence labels in the input file, so every label must be a valid file name for your operating system, otherwise the command will fail.

Example

usearch -fasta_explode db.fa