<< CD-HIT analysis
<< Comparing USEARCH and CD-HIT
How to generate an alignment using CD-HIT
To review the
alignment for a given pair, you can re-compile CD-HIT with the MAKEALIGN
macro defined. Then run CD-HIT with the lowest permitted cutoff, e.g.:
cd-hit-est -i pair.fasta -o pair -c 0.8 If
the pair has 80% or higher identity according to CD-HIT, the alignment
will be written to a file named alignments.txt.
This method applies to CD-HIT v4 at least
through version 4.5.7.
How to generate an alignment using
USEARCH
USEARCH offers a rich set of options for
reviewing alignments and derived values (%id, E-value etc.), including
--uc, --blastout, --blast6out, --fastapairs and --userout. CD-HIT
provides only the %id of a hit via the .clstr output file.
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