Make a UPGMA tree from a
multiple alignment:
muscle -maketree -in seqs.afa -out seqs.phy
Make a
Neighbor-Joining
tree from a multiple alignment:
muscle -maketree -in seqs.afa -out seqs.phy
-cluster neighborjoining
UPGMA or Neighbor joining?
Neighbor-joining trees will usually be a better estimate of the true
phylogenetic tree. UPGMA is faster, which can be useful with large datasets when
N-J is too slow.
Input file
Specified using the -in option. Must be in aligned FASTA
format.
Output file
Specified using the -out option. The tree is written in
Newick format, which is
supported by most phylogenetic analysis packages such as
PHYLIP. See
also this
article on the Newick format in the PHYLIP documentation.
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